QUALITATIVE AND QUANTITATIVE ANALYSIS OF MONOCYTE TRANSENDOTHELIAL MIGRATION BY CONFOCAL MICROSCOPY AND THREE-DIMENSIONAL IMAGE RECONSTRUCTION

Author(s):  
ANUSKA V. ANDJELKOVIC ◽  
MICHAL R. ZOCHOWSKI ◽  
FRANK MORGAN ◽  
JOEL S. PACHTER
Author(s):  
Homero Jiménez-Rabiela ◽  
Benjamín Vázquez-González ◽  
José Luis Ramírez-Cruz ◽  
Pedro García-Segura

Objectives: In this work we show different simulated mechanisms virtually, their objective is to facilitate the qualitative and quantitative analysis of their kinematics; allowing to determine degrees of freedom, relation of times, mechanical advantage, mobility, positions, speeds and accelerations. Such an objective is achieved by simulating them for different positions using discretization and variable timing. Methodology: It consists of files of mechanisms with the corresponding files of links and electronic spreadsheets. The student must modify the dimensional, geometric and kinematic restrictions; remove them and install new ones; to observe the behavior of the mechanism as a system or of the links as integral parts. Contribution: Simplify the cognitive process of the kinematics of the mechanisms. It facilitates the understanding of the Euler and the Lagrange approaches. It allows to understand and evaluate the positions, speeds and accelerations; absolute, relative and apparent. The files of mechanisms and links are virtual, three-dimensional and allow their animation. Electronic spreadsheets control the restrictions of both the mechanisms and their links.


Author(s):  
R. A. Crowther

The reconstruction of a three-dimensional image of a specimen from a set of electron micrographs reduces, under certain assumptions about the imaging process in the microscope, to the mathematical problem of reconstructing a density distribution from a set of its plane projections.In the absence of noise we can formulate a purely geometrical criterion, which, for a general object, fixes the resolution attainable from a given finite number of views in terms of the size of the object. For simplicity we take the ideal case of projections collected by a series of m equally spaced tilts about a single axis.


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